Recombinant CD3 epsilon anticorps

N° du produit ABIN7487698

Cet anticorps anti-CD3 epsilon est un anticorps Lapin Chimeric détectant CD3 epsilon dans FACS et IHC. Adapté pour Porc.

Aperçu rapide pour Recombinant CD3 epsilon anticorps (ABIN7487698)

Antigène: CD3 epsilon (CD3E)

Type d'anticorp: Recombinant Antibody

Reactivité: Porc

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 898H2-6-15

Détail du produit anti-CD3 epsilon anticorps

Fonction: Anti-CD3e [898H2-6-15], Rabbit IgG, kappa

Specificité: Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods.

Isotype: IgG kappa

Information d'application

Indications d'application: The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor-associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Sujet: Leu4/T3, Cluster of Differentiation 3 epsilon chain, T cell glycoprotein CD3 epsilon chain

UniProt: Q7YRN2

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway